Departamento de Física

Facultad de Ciencia
Universidad de Santiago de Chile

Laboratorio de Biofísica

Páginas Laboratorio de Biofísica

Director laboratorio:
Roberto Bernal
Ubicación laboratorio:
Block C
Teléfono:
(56-2)27 181 379
Email laboratorio:
roberto.bernal@usach.cl

Descripción

Cuticle characterization

The high variability of results in uniaxial tensilemechanical test, permeability and optical properties, for samples of the same spicies, are mainly due to the heterogeneity in thickness distribution of the cellulose present in the cuticle. To address this problem, three-dimensional reconstruction of confocal planes of cuticles are used. 

Biocoating and mechanical properties of Red Blood Cells

Adhesion of red blood cells onto functionalized surfaces, creates biological coatings  in a simple and extremely reproductible. During the adhesion process, we observe a "wetting" phenomena, which resembles a drop of fluid onto a solid substrate. The "wetting" process depends not only of thee surface interactions but membrane mechanical properties as well. 

Lipidic membranes characterization

By using Atomic Force and Optical Microscopy techniques, we have developed an easy procedure to evaluate mechanical properties of Giant Bilayer Vesicles. With these tools, we obtain thickness values of bilayer membranes under AFM on Tapping mode. Whereas curvature fluctuation are computed from Phase Constrast microscopy imaging. Then, by tuning the osmotic pressure, elastic properties are obtained. 

Cytoskeletal dynamics

The mechanical response of f-actin stress bundles of RPE-1 cells is under scope when mechanical stretch is applied. Cells are plated onto a H-like PLL-g-PEG/Fibronectin micro patterns on thin PDMS substrates and fluorescent corrected intensity images are used to obtain reliable data of the response of actin structures under mechanical solicitation.

Publicaciones del laboratorio

2015

Time-resolved neurite mechanics by thermal fluctuation assessments
F. Garate, T. Betz, M. Pertusa, R. Bernal
Physical Biology, Vol. 12(6), pp. 66020 doi:10.1088/1478-3975/12/6/066020

2011

Elastic response and wrinkling onset of curved elastic membranes subjected to indentation test
R. Bernal, C. Tassius, F. Melo, J. Ch. Geminard
European Physical Journal E, Vol. 34(2), pp. 13

2010

"Drag force as a tool to test the active mechanical response of PC12 neurites"
R. Bernal, F. Melo, P. A. Pullarkat
Biophysical Journal, Vol. 98, pp. 515 , 523

2007

"Mechanical characterization of elastic membranes: Cell mechanics applications"
R. Bernal, C. Tassius, F. Melo, J. C. Geminard
Applied Physics Letters, Vol. 90, pp. 63903
"Mechanical properties of axon"
R. Bernal, P. A. Pullarkat, F. Melo
Physical Review Letters, Vol. 99, pp. 18301
The Mechanics of Tip Growth Morphogenesis: What we have learned from rubber balloons
R. Bernal, E. R. Rojas, J. Dumais
Journal of Mechanics of Materials and Structures, Vol. 2(6), pp. 1157 , 1168

2004

"Wrinkle formations in axi-symmetrically stretched membranes"
J.Ch. Geminard, R. Bernal, F. Melo
European Physical Journal E, Vol. 15, pp. 117 , 126

2002

Normal-Mode Vortex Interactions
R. Bernal, C. Coste, F. Lund, F. Melo
Physical Review Letters, Vol. 89(3), pp. 34501

Proyectos del Laboratorio

2013

Fondef

National Standards for Shortwave radiation and for UV doses
Director
R. Cordero
Investigadores principales
R. Bernal
Número de proyecto:
IT13I10034
Año de término:
2015

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