Laboratorio de Biofísica
The high variability of results in uniaxial tensilemechanical test, permeability and optical properties, for samples of the same spicies, are mainly due to the heterogeneity in thickness distribution of the cellulose present in the cuticle. To address this problem, three-dimensional reconstruction of confocal planes of cuticles are used.
Biocoating and mechanical properties of Red Blood Cells
Adhesion of red blood cells onto functionalized surfaces, creates biological coatings in a simple and extremely reproductible. During the adhesion process, we observe a "wetting" phenomena, which resembles a drop of fluid onto a solid substrate. The "wetting" process depends not only of thee surface interactions but membrane mechanical properties as well.
Lipidic membranes characterization
By using Atomic Force and Optical Microscopy techniques, we have developed an easy procedure to evaluate mechanical properties of Giant Bilayer Vesicles. With these tools, we obtain thickness values of bilayer membranes under AFM on Tapping mode. Whereas curvature fluctuation are computed from Phase Constrast microscopy imaging. Then, by tuning the osmotic pressure, elastic properties are obtained.
The mechanical response of f-actin stress bundles of RPE-1 cells is under scope when mechanical stretch is applied. Cells are plated onto a H-like PLL-g-PEG/Fibronectin micro patterns on thin PDMS substrates and fluorescent corrected intensity images are used to obtain reliable data of the response of actin structures under mechanical solicitation.